Typical flow velocity for Superdex pg is 10 to 50 cm/h. However, flow velocity and sample feed must be optimized for each separation method to ensure maximum productivity (see Section 6.) The pressure/flow velocity curves are shown in Figure 2. Superdex pg K av 10 MW 1 Superdex 20% ethanol (for HiLoad Superdex 30 pg and Superdex 75 pg, use mM sodium acetate in 20% ethanol). Note: Use a lower flow rate for viscous 20% ethanol. Store the column in 20% ethanol to prevent any microbial growth. Connect the transport tool to the column outlet, to prevent air entering the column. Fill the transport tool up to. Superdex Increase 5/ GL is for rapid size analysis of proteins and other biomolecules. Short cycle times, together with small sample volume and low consumption of buffer make this column ideal for use in screening experiments to check protein homogeneity. Superdex Increase 10/ GL is suitable for small scale.
Superdex 10/ GL and and 5/ GL have been discontinued and are replaced by Superdex Increase GL columns. Please change the country on your profile in order to switch to another country store. Superdex Increase 5/ GL is for rapid size analysis of proteins and other biomolecules. Short cycle times, together with small sample volume and low consumption of buffer make this column ideal for use in screening experiments to check protein homogeneity. Superdex Increase 10/ GL is suitable for small scale. 20% ethanol (for HiLoad Superdex 30 pg and Superdex 75 pg, use mM sodium acetate in 20% ethanol). Note: Use a lower flow rate for viscous 20% ethanol. Store the column in 20% ethanol to prevent any microbial growth. Connect the transport tool to the column outlet, to prevent air entering the column. Fill the transport tool up to.
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